微生物培養基(中英文對照 其中有很多配方適合食用菌培養) 質量單位:g(克)
THE COMPOSITION OF MEDIA
培養基及成分
1. Acetobacter Medium (醋酸菌培養基)
Glucose (葡萄糖) 100g Yeasst extract (酵母膏) 10g
CaCO3 20g Agar (瓊脂) 15g
Distilled water (蒸餾水) 1000ml Adjust (調) pH to 6.8
適用范圍:惡臭醋酸桿菌混濁變種
2. Nutrient Agar (營養肉汁瓊脂)
Pepton (蛋白胨) 5g Beef extract (牛肉膏) 30g
NaCl 5g Agar (瓊脂) 15g
Distilled water (蒸餾水) Adjust (調) pH to 7.0-7.2
[Note]:When cultivation of Bacillus,5mg of to MnSO4.H2O may be added .
It is favorable to promote spore formation .
適用范圍:產氣氣桿菌、糞產堿桿菌、蠟狀芽孢桿菌、蠟狀芽孢桿菌蕈狀變種、地衣形芽孢桿菌、巨大芽孢桿菌、多粘芽孢桿菌、塵埃芽孢桿菌、短小芽孢桿菌、嗜熱脂肪芽孢桿菌、枯草芽孢桿菌、枯草芽孢桿菌深黑變種、蘇云金芽孢桿菌、蘇云金芽孢桿菌蠟螟亞種(青蟲菌)、蘇云金芽孢桿菌戈爾斯德變種、蘇云金芽孢桿菌猝倒亞種、產氨短桿菌、黃色短桿菌、谷氨酸棒狀桿菌、北京棒桿菌、大腸埃希氏菌(大腸桿菌)、銅綠假單胞菌(綠膿桿菌)、凸形假單胞桿菌、熒光假單胞菌、彎曲假單胞菌、惡臭假單胞菌、假單胞桿菌、藤黃八疊球菌、亞黃八疊球菌、尿素八疊球菌、金黃色葡萄球菌、運動發酵單孢菌
3. Azotobacter Medium (固氮菌培養基)
KH2PO4 0.2g K2HPO4 0.8g
MgSO4.7H2O 0.2g CaSO4.2H2O 0.1g
Na2MoO4.2H2O Trace(微量) Yeast axtract(酵母膏) 0.5g
Mannitol(甘露醇) 20g FeCl3 Tract(微量)
Distilled water (蒸餾水) 1000ml Agar (瓊脂) 15g
Adjust (調) pH to 7.2
適用范圍:固氮菌、膠質芽孢桿菌
4. Corn Meal Medium (玉米粉培養基)
Maize flour (玉米粉) 5g Peptone (蛋白胨) 0.1g
Glucose (葡萄糖) 1g Tap water (自來水) 1000ml
[Note]:Boil the mixture in autoclave at 121℃ for 1 hr. distribute the medium into 18ⅹ18 mm tubes , each contains 10 ml of the liquid , then autoclave at 121℃ for 1 hr . again (15磅蒸煮1小時,分裝入18ⅹ18毫米試管,每管深度達6厘米。15磅再次滅菌15小時。)
5. Lactic-bacteria Medium I (乳酸菌培養基 I )
Yeast extract (酵母膏) 7.5g Peptone (蛋白胨) 7.5g
Glucose (葡萄糖) 10g KH2PO4 2g
Tomato juice (西紅柿汁) 100ml Tween (吐溫) 80 0.5ml
Distilled water (蒸餾水) 900ml pH 7.0
適用范圍:植物乳桿菌(胚芽乳桿菌)、嗜熱乳酸鏈球菌
6. Lactic-bacteria Midium Ⅱ (乳酸菌培養基 Ⅱ)
Lacto-casein peptone (乳酪蛋白胨) 10g Beef extract (蛋白胨) 10g
Yeast extract (酵母膏) 5g Glucose (葡萄糖) 5g
Tween (吐溫) 80 1g K2HPO4 2g
Na-acetate (醋酸鈉) 5g Diamine citrate (檸檬酸二胺) 2g
MgSO4.7H2O 0.2g MnSO4.H2O 0.05g
Distilled water (蒸餾水) 1000m pH 6.5-6.8
適用范圍:植物乳桿菌(胚芽乳桿菌)
7. Peotone Glucose Yeast extract Medium PGY (蛋白胨、酵母膏、葡萄糖培養基)
Peptone(蛋白胨) 10g Yeast extract (酵母膏) 5g
Glucose (葡萄糖) 1g Distilled water (蒸餾水) 1L
8. Glycerol Agar (甘油瓊脂)
Peptone (蛋白胨) 5g Beef extract (酵母膏) 3g
Glycerol (甘油) 20g Top water (自來水) 1000ml
Agar (瓊脂) 15g pH 7.0-7.2
9. Rhizobium medium (根瘤菌培養基)AS 9
Yeast eztract (酵母膏) 1g Soil eztract (土壤浸提液) 200ml
Mannitol (甘露醇) 10g Agar (瓊脂) 15g
Distilled water (蒸餾水) 800ml pH 7.2
[Note]:Soil extract:Suspend 50g finely and dried gardon soil in 200ml of tap water. Autoclave at 121℃ for 1 hr. Decant through cotton-cloth, filler though paper, make up volume to 200ml. Resterilize for 20 minutes at 121℃ , then mixed with othringredients and distributed. (土壤浸提液的制法:取土壤50克,加水200毫升,15磅蒸煮1小時,經濾紙過濾后加水補足到200毫升。)
適用范圍:大豆根瘤菌(慢生型)、豇豆慢生根瘤菌、花生根瘤菌、紫云英根瘤菌、
大豆根瘤菌(快生型)、大豆根瘤菌、豌豆根瘤菌、苜蓿根瘤菌、田菁根瘤菌
10. Mannitol Agar (甘露醇瓊脂)
Yeast extract (酵母膏) 5g Peptone (蛋白胨) 3g
Mannitol (甘露醇) 25g Agar (瓊脂) 15g
Distilled water (蒸餾水) 1000ml